Y-Haplogroups in American Whites and Blacks

ASHG 2003, Poster #1594

Determination of Y-Haplogroups Utilizing Suspension Arrays.

D. Carlson et al.

The purpose of this study was to develop a method for rapid determination of Y-haplogroup of DNA samples. Forty-twoY-chromosome single nucleotide polymorphisms (SNPs) were selected based on their association with a particular ancestral haplogroup. Each polymorphic region was amplified in a multiplexed PCR reaction utilizing primers labeled with a fluorescent reporter dye. Oligonucleotides specific for each allele in the multiplex were immobilized on the surface of latex microspheres containing fluorescent identifiers. Labeled PCR products were then hybridized to the microspheres in multiplexed mode. Reporter dyes and identifier dyes were read simultaneously for each microsphere in a flow analyzer. Allele-calling software (MasterPlexGT, MiraiBio) automated the genotyping. The flow reader required 1.5 hr to analyze 96 wells of multiplexed reactions. The most frequent Y-haplogroups were found to be E1a for African Americans and R1b for U.S. Caucasians. The percentage of admixture of Caucasian Y-haplogroups in the African American samples was slightly more than 30%. Frequency of African markers in the Caucasian samples was less than 5%.